%0 Journal Article %T Rapid cooling of rabbit embryos in a synthetic medium %+ VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS) %+ Interactions Cellules Environnement - UR (ICE) %+ ISARA-Lyon %A Teixeira, Magda %A Commin, Loris %A Gavin-Plagne, Lucie %A Bruyère, Pierre %A Buff, S. %A Joly, Thierry %< avec comité de lecture %@ 0011-2240 %J Cryobiology %I Elsevier %V 85 %P 113-119 %8 2018-12 %D 2018 %R 10.1016/j.cryobiol.2018.07.006 %Z Life Sciences [q-bio]/Animal biology/Veterinary medicine and animal Health %Z Life Sciences [q-bio]/Biotechnology %Z Life Sciences [q-bio]/Agricultural sciences/ZootechnyJournal articles %X Embryo cryopreservation media usually contain animal-derived products, such as bovine serum albumin (BSA). These products present two major disadvantages: an undefined variable composition and a risk of pathogen transmission. We aimed to evaluate the effect of replacing BSA in rabbit embryo rapid cooling “freezing” and warming media with a chemically defined medium with no animal-derived products: STEM ALPHA. Cryo3 (“Cryo3”).A total of 1540 rabbit morulae were divided into three cryopreservation groups (group 1: BSA, group 2: 20% Cryo3 and group 3: 100% Cryo3) and a fresh controls group. After rapid cooling, embryos were cultured (in vitro approach), or transferred into synchronized does (in vivo approach). In the in vitro approach, post-warm survival rates obtained with 100% Cryo3 (94.9%) were superior to BSA (90.8%) and 20% Cryo3 (85.6%). The blastocyst formation rate was similar between BSA, 20% Cryo3 and 100% Cryo3 groups (85.1, 77.9 and 83.3%, respectively), as was the expansion/hatching rate (63.1, 63.4 and 58.0%, respectively) and embryo mitochondrial activity. In the in vivo approach, pregnancy (80.0, 68.0 and 95.2%, respectively), implantation (40.5, 45.9 and 44.8%, respectively), and live-foetus rates (35.6, 35.5 and 38.1%, respectively) were similar between the three groups. To conclude, Cryo3 can replace BSA in rabbit embryo rapid cooling “freezing” and warming media. %G English %2 https://vetagro-sup.hal.science/hal-03175587/document %2 https://vetagro-sup.hal.science/hal-03175587/file/Rapid_cooling_of_rabbit_embryos_in_a_synthetic_medium_2018.pdf %L hal-03175587 %U https://vetagro-sup.hal.science/hal-03175587 %~ VETAGRO-UPSP-ICE %~ VETAGRO-SUP %~ UDL %~ UNIV-LYON %~ ISARA %~ TEST3-HALCNRS