Controlled Contact between Beads and Cells for the Characterization of Receptor–Ligand Bonds
Résumé
The controlled contact between two micro-sized objects, such as
beads and cells, and the assessment of their adhesion status is
demonstrated in this research. The controlled contact is carried
out in a microfluidic channel under flow conditions and makes use
of a combination of hydrodynamic traps, flow drag force and
dielectrophoretic (DEP) force to maintain the two objects in
contact for the desired duration in a first step. Then, the pair
objects are separated in the second step in order to explore their
adhesion status. Adhesion events are mediated by the bond formed
between a receptor and its ligand, and their binding kinetic
parameters can be extracted from the measurements using the
proposed device.
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