Multi-Spectral Reflection Matrix for Ultra-Fast 3D Label-Free Microscopy - Institut Langevin
Article Dans Une Revue Nature Photonics Année : 2024

Multi-Spectral Reflection Matrix for Ultra-Fast 3D Label-Free Microscopy

Résumé

Label-free microscopy exploits light scattering to obtain a three-dimensional image of biological tissues. However, light propagation is affected by aberrations and multiple scattering, which drastically degrade the image quality and limit the penetration depth. Multi-conjugate adaptive optics and time-gated matrix approaches have been developed to compensate for aberrations but the associated frame rate is extremely limited for 3D imaging. Here we develop a multi-spectral matrix approach to solve these fundamental problems. Based on a sparse illumination scheme and an interferometric measurement of the reflected wave-field at multiple wavelengths, the focusing process can be optimized in post-processing for any voxel by addressing independently each frequency component of the reflection matrix. A proof-of-concept experiment demonstrates the three-dimensional image of an opaque human cornea over a 0.1 mm^3-field-of-view at a 290 nm-resolution and a 1 Hz-frame rate. This work paves the way towards a fully-digital microscope allowing real-time, in-vivo, quantitative and deep inspection of tissues
Fichier principal
Vignette du fichier
main.pdf (26.93 Mo) Télécharger le fichier
Origine Fichiers produits par l'(les) auteur(s)

Dates et versions

hal-04211838 , version 1 (20-09-2023)
hal-04211838 , version 2 (17-10-2023)
hal-04211838 , version 3 (11-09-2024)

Licence

Identifiants

Citer

Paul Balondrade, Victor Barolle, Nicolas Guigui, Emeric Auriant, Nathan Rougier, et al.. Multi-Spectral Reflection Matrix for Ultra-Fast 3D Label-Free Microscopy. Nature Photonics, 2024, ⟨10.1038/s41566-024-01479-y⟩. ⟨hal-04211838v3⟩
73 Consultations
52 Téléchargements

Altmetric

Partager

More